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Extra Credit [v0.5 ALPHA] [APK]



(2) The tailings or wastes produced by the extraction or concentration of uranium or thorium from ore processed primarily for its source material content, including discrete surface wastes resulting from uranium solution extraction processes. Underground ore bodies depleted by these solution extraction operations do not constitute "byproduct material" within this definition;




Extra Credit [v0.5 ALPHA] [APK]



Radiation (ionizing radiation) means alpha particles, beta particles, gamma rays, x-rays, neutrons, high-speed electrons, high-speed protons, and other particles capable of producing ions. Radiation, as used in this part, does not include non-ionizing radiation, such as radio- or microwaves, or visible, infrared, or ultraviolet light.


Working level (WL) is any combination of short-lived radon daughters (for radon-222: polonium-218, lead-214, bismuth-214, and polonium-214; and for radon-220: polonium-216, lead-212, bismuth-212, and polonium-212) in 1 liter of air that will result in the ultimate emission of 1.3x105 MeV of potential alpha particle energy.


(a) The criteria in this subpart apply to the decommissioning of facilities licensed under parts 30, 40, 50, 52, 60, 61, 63, 70, and 72 of this chapter, and release of part of a facility or site for unrestricted use in accordance with 50.83 of this chapter, as well as other facilities subject to the Commission's jurisdiction under the Atomic Energy Act of 1954, as amended, and the Energy Reorganization Act of 1974, as amended. For high-level and low-level waste disposal facilities (10 CFR parts 60, 61, and 63), the criteria apply only to ancillary surface facilities that support radioactive waste disposal activities. The criteria do not apply to uranium and thorium recovery facilities already subject to appendix A to 10 CFR part 40 or the uranium solution extraction facilities.


PrBoom also supports playing Doom add-on levels, called PWADs, which are small extra .wad files which just contain extra levels or other resources. PWADs are ONLY ADD-ONS, you still need the original IWADthat they are designed to work with. In practice, most PWADs on the Internet require doom2.wad (although some work with doom.wad).


If you are thinking on participating in OPW as an intern, please take a look at our OPW wiki page for some initial guidelines. The page is still a work in progress, but there should be enough information there to get you started. If you, on the other hand, are thinking on sponsoring work on FFmpeg through the OPW program, please get in touch with us at opw@ffmpeg.org. With your help, we might be able to secure some extra intern spots for this round!


However, as shown in Figure 7b, if these seven points are plotted on a graph of 1/velocity against 1/substrate concentration (i.e. a double-reciprocal plot), the data are linearized, and the line can be easily extrapolated to the left to provide intercepts on both the y-axis and the x-axis, from which Vmax and Km, respectively, can be evaluated.


A further advantage of using microbial enzymes is their ease of extraction. Many of the microbial enzymes used in biotechnological processes are secreted extracellularly, which greatly simplifies their extraction and purification. Microbial intracellular enzymes are also often easier to obtain than the equivalent animal or plant enzymes, as they generally require fewer extraction and purification steps.


Animal and plant sources usually need to be transported to the extraction facility, whereas when microorganisms are used the same facility can generally be employed for production and extraction. In addition, commercially important animal and plant enzymes are often located within only one organ or tissue, so the remaining material is essentially a waste product, disposal of which is required.


Bacterial enzymes were developed in France by August Boidin and Jean Effront, who in 1913 found that Bacillus subtilis produced a heat-stable α-amylase when grown in a liquid medium made by extraction of malt or grain. The enzyme was primarily used within the textile industry for the removal of the starch that protects the warp in the manufacture of cotton.


Simple corn syrups can be manufactured by breaking down starch derived from corn using the enzyme glucoamylase alone or in combination with α-amylase. These enzymes are cheap and can be used in a soluble form. Since starch has to be extracted from corn at high temperatures (because starch has poor solubility at low temperatures and forms very viscous solutions), the process utilizes enzymes from thermophilic organisms, which have very high temperature optima. Simple corn syrup is therefore composed predominantly of glucose, which unfortunately has only 75% of the sweetness of sucrose. However, in order to make the syrup sweeter the enzyme glucose isomerase, which catalyses the following reaction, can be employed:


The GM24385 (RRID:CVCL_1C78) EBV-immortalized LCL of HG002 was obtained from the National Institute for General Medical Sciences (NIGMS) Human Genetic Cell Repository at the Coriell Institute for Medical Research. This cell line was used to generate the Oxford Nanopore sequencing and Bionano mapping data. For the Illumina and Pacific Biosciences sequencing data, NIST Reference Material (RM) 8391 DNA was used, which was prepared from a large batch of GM24385 to control for differences arising during cell growth. For paternal (HG003) and maternal (HG004) samples, DNA was extracted from cell lines publicly available as GM24149 (RRID:CVCL_1C54) and GM24143 (RRID:CVCL_1C48), respectively, and Illumina sequencing of DNA from NIST RM 8392 (containing vials of HG002, HG003 and HG004) was used.


The sequence data used for this study (HG002 Data Freeze v1.0) are available on GitHub ( -pangenomics/HG002_Data_Freeze_v1.0). DNA samples were extracted from large homogenized growths of B lymphoblastoid cell lines of HG002, HG003 and HG004 from the Coriell Institute for Medical Research.


MaSuRCA v3.3.1 (ref. 32) with default parameters was run on the combined Illumina, ONT and PacBio HiFi data to obtain a set of contigs designated the Ash1 v0.5 assembly. The ONT and PacBio data were an earlier release, from 2018, and the read lengths were shorter than the later release used by most other methods in this evaluation. After initial scaffolding, MaSuRCA was used to remove redundant haplotype-variant scaffolds by aligning the assembly to itself and looking for scaffolds that were completely covered by another larger scaffold and that were more than 97% identical to the larger scaffold. To scaffold and assign the assembled contigs to chromosomes, we used a reference-based scaffolding method embodied in the chromosome_scaffolder script included in MaSuRCA. We used the GRCh38.p12 as the reference (without the ALT scaffolds), and we enabled an option in chromosome_scaffolder that allows it to fill in gaps with the GRCh38 sequence, using lowercase letters. Finally, we examined SNVs reported at high frequency in an Ashkenazi population from the Genome Aggregation Database (gnomAD). GnomAD v3.0 contains SNV calls from short-read whole-genome data from 1,662 Ashkenazi individuals. At 273,866 heterozygous SNV sites where HG002 contained the Ashkenazi major allele and where our assembly used a minor allele, we replaced the allele in Ash1 with the Ashkenazi major allele. A publication of the final curated asm1 assembly has been made60. Compute time was not tracked. The source code is available ( ).


All scaffolds of the final HG002 maternal and paternal assemblies were aligned by minimap2 to the T2T-CHM13 reference and the Y chromosome of GRCh38. Some of the contigs within the HG002 scaffolds had alternate alignments to CHM13, which we did not include in the analyses to avoid the ambiguity. The phase density of contigs were calculated using the parental short reads. We then extracted haplotype-specific k-mers from contigs and determined the colour value by the number of these k-mers.


You can enable the virtual camera in VSeeFace, set a single colored background image and add the VSeeFace camera as a source, then going to the color tab and enabling a chroma key with the color corresponding to the background image. Note that this may not give as clean results as capturing in OBS with proper alpha transparency.


If supported by the capture program, the virtual camera can be used to output video with alpha transparency. To make use of this, a fully transparent PNG needs to be loaded as the background image. Starting with version 1.13.25, such an image can be found in VSeeFace_Data\StreamingAssets. Partially transparent backgrounds are supported as well. Please note that using (partially) transparent background images with a capture program that do not support RGBA webcams can lead to color errors. OBS supports ARGB video camera capture, but require some additional setup. Apparently, the Twitch video capturing app supports it by default.


We are introducing Release channels, a new way to keep your GKE clusters up to date. The Rapid release channel is available, and includes v1.14.1-gke.5 (alpha). You can sign up to try release channels and preview GKE v1.14.x.


To create a cluster using this version, use the followingcommand, replacing my-alpha-cluster with the nameof your cluster. Use the exact cluster version provided in the command. You canadd other configuration options, but do not change any of the ones below.


The Container-Optimized OS with containerd node image has been upgraded fromcos-69-10895-138-0-c123tocos-69-10895-138-0-c124for clusters runningKubernetes 1.12.5-gke.10+ and alpha clusters running Kubernetes 1.13+.


A new `cos_containerd` image is now available and set by default for trying out the containerd integration in the alpha clusters running Kubernetes 1.10.4-gke.0 and above. See the containerd runtime alpha user guide for more information, or learn about the containerd integration in the recent Kubernetes blog post. 041b061a72


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